Production Of Recombinant BMP7 In Bioreactors (Record no. 16613)

MARC details
000 -LEADER
fixed length control field 01924nam a22001937a 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 660.6
Item number B575P
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Bhanwariya, Manju
245 ## - TITLE STATEMENT
Title Production Of Recombinant BMP7 In Bioreactors
Statement of responsibility, etc by Manju Bhanwariya
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication IIT Jodhpur
Name of publisher Department of Chemical Engineering
Year of publication 2023
300 ## - PHYSICAL DESCRIPTION
Number of Pages viii, 35p.
500 ## - GENERAL NOTE
General note The development of the kidneys and eyes are biological processes that are influenced by bone morphogenetic protein (BMP7), a secreted homo-dimeric glycoprotein, and a growth factor which belongs to the Transforming Growth Factor Beta (TGFβ) superfamily. As a result, it might also have further therapeutic promise for treating a number of illnesses. One of the microorganisms that is most frequently employed for the commercial production of heterologous proteins is Saccharomyces cerevisiae. In our study, BMP7 is expressed using S. cerevisiae in a shake flask. Shake-flask fermentation is a method that allows for continuous shaking of a liquid culture at a specific constant temperature. The constant shaking motion helps the microbe grow consistently and absorb nutrients equally while using a minimal amount of oxygen. The S. cerevisiae alpha mating factor signal peptide is commonly used to control the secretion of multiple diverse heterologous proteins in a variety of hosts like Pichia pastoris. Therefore, to secrete BMP7 protein for easier separation from the extracellular medium, the coding sequence of BMP7 is linked to the alpha mating factor secretion signal peptide and produced in a 100 ml shake flask. As future work, BMP7 may be produced in a 1-liter bioreactor.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Chemical Engineering
Topical Term Bone Morphogenetic Protein (BMP7)
Topical Term Saccharomyces cerevisiae
Topical Term Alpha Mating Factor Signal Peptide
Topical Term MTech Theses
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Sarkar, Prasenjit
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Book
Holdings
Withdrawn status Lost status Damaged status Not for loan Collection code Permanent Location Current Location Shelving location Date acquired Source of acquisition Full call number Accession Number Price effective from Koha item type
        Theses S. R. Ranganathan Learning Hub S. R. Ranganathan Learning Hub Reference 2024-04-01 Office of Academics 660.6 B575P TM00548 2024-07-02 Thesis