Production Of Recombinant BMP7 In Bioreactors (Record no. 16613)
[ view plain ]
000 -LEADER | |
---|---|
fixed length control field | 01924nam a22001937a 4500 |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 660.6 |
Item number | B575P |
100 ## - MAIN ENTRY--AUTHOR NAME | |
Personal name | Bhanwariya, Manju |
245 ## - TITLE STATEMENT | |
Title | Production Of Recombinant BMP7 In Bioreactors |
Statement of responsibility, etc | by Manju Bhanwariya |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Place of publication | IIT Jodhpur |
Name of publisher | Department of Chemical Engineering |
Year of publication | 2023 |
300 ## - PHYSICAL DESCRIPTION | |
Number of Pages | viii, 35p. |
500 ## - GENERAL NOTE | |
General note | The development of the kidneys and eyes are biological processes that are influenced by bone morphogenetic protein (BMP7), a secreted homo-dimeric glycoprotein, and a growth factor which belongs to the Transforming Growth Factor Beta (TGFβ) superfamily. As a result, it might also have further therapeutic promise for treating a number of illnesses. One of the microorganisms that is most frequently employed for the commercial production of heterologous proteins is Saccharomyces cerevisiae. In our study, BMP7 is expressed using S. cerevisiae in a shake flask. Shake-flask fermentation is a method that allows for continuous shaking of a liquid culture at a specific constant temperature. The constant shaking motion helps the microbe grow consistently and absorb nutrients equally while using a minimal amount of oxygen. The S. cerevisiae alpha mating factor signal peptide is commonly used to control the secretion of multiple diverse heterologous proteins in a variety of hosts like Pichia pastoris. Therefore, to secrete BMP7 protein for easier separation from the extracellular medium, the coding sequence of BMP7 is linked to the alpha mating factor secretion signal peptide and produced in a 100 ml shake flask. As future work, BMP7 may be produced in a 1-liter bioreactor. |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical Term | Department of Chemical Engineering |
Topical Term | Bone Morphogenetic Protein (BMP7) |
Topical Term | Saccharomyces cerevisiae |
Topical Term | Alpha Mating Factor Signal Peptide |
Topical Term | MTech Theses |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Sarkar, Prasenjit |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Book |
Withdrawn status | Lost status | Damaged status | Not for loan | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Source of acquisition | Full call number | Accession Number | Price effective from | Koha item type |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Theses | S. R. Ranganathan Learning Hub | S. R. Ranganathan Learning Hub | Reference | 2024-04-01 | Office of Academics | 660.6 B575P | TM00548 | 2024-07-02 | Thesis |