000			01924nam a22001937a 4500
082			_a660.6 _bB575P
100			_aBhanwariya, Manju _945609
245			_aProduction Of Recombinant BMP7 In Bioreactors _cby Manju Bhanwariya
260			_aIIT Jodhpur _bDepartment of Chemical Engineering _c2023
300			_aviii, 35p.
500			_aThe development of the kidneys and eyes are biological processes that are influenced by bone morphogenetic protein (BMP7), a secreted homo-dimeric glycoprotein, and a growth factor which belongs to the Transforming Growth Factor Beta (TGFβ) superfamily. As a result, it might also have further therapeutic promise for treating a number of illnesses. One of the microorganisms that is most frequently employed for the commercial production of heterologous proteins is Saccharomyces cerevisiae. In our study, BMP7 is expressed using S. cerevisiae in a shake flask. Shake-flask fermentation is a method that allows for continuous shaking of a liquid culture at a specific constant temperature. The constant shaking motion helps the microbe grow consistently and absorb nutrients equally while using a minimal amount of oxygen. The S. cerevisiae alpha mating factor signal peptide is commonly used to control the secretion of multiple diverse heterologous proteins in a variety of hosts like Pichia pastoris. Therefore, to secrete BMP7 protein for easier separation from the extracellular medium, the coding sequence of BMP7 is linked to the alpha mating factor secretion signal peptide and produced in a 100 ml shake flask. As future work, BMP7 may be produced in a 1-liter bioreactor.
650			_aDepartment of Chemical Engineering _945610
650			_aBone Morphogenetic Protein (BMP7) _945611
650			_aSaccharomyces cerevisiae _945612
650			_aAlpha Mating Factor Signal Peptide _945613
650			_aMTech Theses _945614
700			_aSarkar, Prasenjit _945615
942			_cBK
999			_c16613 _d16613