000 01924nam a22002057a 4500
082 _a570.2
_bM214F
100 _aMaheshwari, Khushi
_945541
245 _aFabrication of Coliform and Biological Oxygen Demand Sensor
_cby Khushi Maheshwari
260 _aIIT Jodhpur
_bDepartment of Bioscience and Bioengineering
_c2023
300 _avii,46p.
_bHB
500 _aPollution in water resources is rising high due to over-exploitation and lack of purity and freshness around water bodies, which includes dumping of organic/industrial substances or fecal sludge threatening the human and animal population. Detecting bacterial contamination in water using an electrochemical-based sensor can be an effective approach. Coliform bacteria are abundantly present in wastewater, with Escherichia coli being one of the major types. Graphene oxide has shown promising results in sensor development. The interaction between graphene oxide and E. coli dilutions from 10^-1 to 10^-10, upon incubating with 3% H2O2 v/v, results in a change in voltage in the source measuring unit. In this study, an electrochemical sensor is prepared using a graphene oxide-coated screen-printed electrode for the detection of catalase-positive E. coli bacteria in water samples based on the breakdown of H2O2. The LOD and LOQ for the electrochemical system were recorded as 2.851 CFU/ml and 8.641 CFU/ml, respectively, with a sensitivity of 5.1467 mV CFU/ml for E. coli. The sensor response time is within milliseconds. These properties make our system efficient and reliable for detecting coliform bacteria in water samples.
650 _aDepartment of Bioscience and Bioengineering
_945542
650 _aElectrochemical sensors
_945543
650 _aColiform bacteria detection
_945544
650 _aEscherichia coli
_945545
650 _aMTech Theses
_945546
700 _aChhabra, Meenu
_945547
700 _aDhanekar, Saakshi
_945548
942 _cTH
999 _c16603
_d16603