This book constitutes the refereed post proceedings of the 16th Research Conference on Metadata and Semantic Research, MTSR 2022, held in London, UK, during November 7-11, 2022. The 21 full papers and 4 short papers included in this book were carefully reviewed and selected from 79 submissions. They were organized in topical sections as follows: metadata, linked data, semantics and ontologies - general session, and track on Knowledge IT Artifacts (KITA), Track on digital humanities and digital curation, and track on cultural collections and applications, track on digital libraries, information retrieval, big, linked, social & open data, and metadata, linked data, semantics and ontologies - general session, track on agriculture, food & environment, and metadata, linked Data, semantics and ontologies - general, track on open repositories, research information systems & data infrastructures, and metadata, linked data, semantics and ontologies - general, metadata, linked data, semantics and ontologies - general session, and track on european and national projects.