Adult neurogenesis is the genesis of new functional neurons in the brain during the adult lifespan through proliferation and differentiation of neural stem cells (NSCs). Under normal conditions, the neurogenesis in adult mammalian brain is reported in two restricted brain regions: the sub ventricular zone (SVZ) of lateral ventricles and the sub granular zone (SGZ) of the dentate gyrus in the hippocampus. Neural stem cell contributes to plasticity of the adult brain and has a role in memory and learning. The different states of neural stem cell subpopulation with distinct molecular profile is identifiable through cell surface markers and intracellular regulators. Single cell RNA sequencing provides an unbiased way of investigating transcriptome profile based cellular heterogeneity among the adult NSC lineage. Our analysis of single cell RNA-sequencing data from Hochgerner et al suggest multiple possible subpopulations within the adult stem cell population called the ‘Radial Glial-like Cell’(RGL), justifying presence of intra-cluster heterogeneity among RGL. Additionally, RGL population is clustered based on age. Considering the expression profile of neurogenic and astrogenic lineage genes, the RGL sub-clusters are sorted for further analysis. The whole transcriptome analysis reports differentially expressed genes among the sorted clusters. To investigate the roles of a specific gene in adult hippocampal neurogenesis, in-vivo genetic lineage tracing is performed using inducible Cre/LoxP mouse model. The Nestin-CreERT2 mice has the advantage of temporal control of genetic recombination and restricted expression of reporter gene in the NSCs. Analysis based on wild-type and knock-out mice, thirty days after deletion of the geneof-interest show depletion of RGL numbers, increased aberrant neurons in the granular zone, and increased numbers of astrocytes, implicated the importance of this gene in adult neurogenesis.